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Vitamin D Blood Test in Pune


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Vitamin D testing measures the level of this essential substance in your blood. It is used to diagnose vitamin D deficiencies or to monitor treatment for a known deficiency.

The main function of vitamin D is to aid in the absorption of calcium during bone formation. Maintaining adequate levels of vitamin D supports healthy bones. In addition, vitamin D has anti-inflammatory and other properties that play a role in maintaining normal muscle, immune, and nervous system functions.

Vitamin D blood test at home can be used to check your levels so a deficiency can be corrected. Less commonly, vitamin D testing may be used to detect vitamin D toxicity, a condition in which there is an excess of vitamin D in the body.

Sunrise Diagnostic offers the best Vitamin D test near me in Pune at an affordable price. 

Purpose of Vitamin D test:

Vitamin D testing is used to determine whether you have enough vitamin D in your body to support normal bodily functions. An inadequate level of vitamin D is called a deficiency. A more mild lack of vitamin D can be referred to as an insufficiency. Your health care provider may order a vitamin D test in order to:

  • Screen for a possible deficiency or insufficiency if you have a risk factor that increases the likelihood of having a low level of vitamin D. A risk factor is anything that makes it more likely for a health problem to occur.
  • Diagnose a vitamin D deficiency or insufficiency if you have symptoms that suggest low levels. Testing may also be used to diagnose a rare vitamin D toxicity due to excess intake of vitamin D supplements.
  • Monitor your vitamin D levels if you are being treated for a previously detected deficiency.

What does the test measure?

Vitamin D blood test at home is necessary for maintaining health. It helps your body absorb minerals like calcium, magnesium, and phosphorus from your diet. Vitamin D also helps maintain proper levels of calcium, phosphate, and parathyroid hormone in your blood.

Vitamin D plays an important role in helping the body absorb calcium, which makes it crucial to maintaining bone health. Inadequate levels of vitamin D can lead to abnormal bone development, bone weakness, and pain.

Unlike other vitamins, Vitamin D can be created in the human body. When your skin is exposed to direct sunlight, ultraviolet radiation converts a chemical in your skin into an active form of vitamin D. Vitamin D can also be obtained through the diet, including from fortified or vitamin-enriched foods and vitamin supplements.

There are two major forms of Vitamin D:

  • Vitamin D2 (ergocalciferol): Vitamin D2 is created in plants, such as yeast or mushrooms. It is also available as a supplement and in fortified foods.
  • Vitamin D3 (cholecalciferol): Vitamin D3 is generated in the skin when it is exposed to sunlight. It is also found in some animal-based foods and may be consumed in certain fortified foods or dietary supplements.

When should you get a vitamin D test?

Vitamin D testing is ordered to determine if a deficiency, insufficiency, or toxic level of vitamin D is present or to monitor treatment for a previously diagnosed deficiency.

Your health care provider may order a vitamin D blood test for you if you are experiencing symptoms of a vitamin D deficiency, such as:

  • Weakening of the bones
  • Abnormal bone development
  • Bone deformity
  • Bone pain
  • Muscle weakness or cramps
  • Seizures
  • Dental abnormalities

Sometimes vitamin D tests are used as screening tests for individuals at increased risk of a vitamin D deficiency. Screening tests are conducted before any symptoms occur. The following are factors that may increase your risk of developing a vitamin D insufficiency or deficiency:

  • Age over 65
  • Obesity
  • History of weight loss surgery
  • Osteoporosis
  • Reduced ability to make vitamin D in the skin due to limited sun exposure, sunscreen use, or dark skin pigmentation
  • Digestive diseases that make it difficult to absorb nutrients from food, including celiac disease and Crohn’s disease
  • Kidney and liver disease
  • Use of certain medications

Vitamin D tests may also be ordered if your health care provider suspects that you may have abnormally high vitamin D levels, known as vitamin D toxicity. This occurs as a result of taking too much vitamin D in supplements rather than from too much sun exposure or dietary intake.

Excess vitamin D in supplement form may cause your body to absorb more calcium from food and to reabsorb calcium from the bones into the blood. This results in excess calcium in the blood, also known as hypercalcemia, which can lead to symptoms like fatigue, confusion, bone pain, nausea and vomiting, frequent urination, and kidney problems.

A healthcare provider who is familiar with your medical history is in the best position to determine whether you might benefit from vitamin D testing.

Both vitamin D2 and D3 need to go through chemical changes before being able to be used by the body. These changes occur in the liver and the kidneys and convert vitamin D into measurable substances called 25-hydroxyvitamin D and 1,25-dihydroxy vitamin D:

  • 25-hydroxyvitamin D: In the liver, vitamin D is converted into 25-hydroxyvitamin D, which is also called 25(OH)D. Most of the time, vitamin D levels will be tested by measuring blood levels of 25(OH)D. Testing 25(OH)D is considered the most accurate way to measure how much vitamin D is in your body because 25(OH)D is the major form of vitamin D circulating in the bloodstream.
  • 1,25-dihydroxy vitamin D: In the kidneys, 25(OH)D is converted into a hormone called active vitamin D or 1,25-dihydroxy vitamin D. Sometimes, health care providers may check your blood level of 1,25 dihydroxyvitamin D, which is also called 1,25(OH)2D.This substance is generally not used to detect inadequate vitamin D levels, but it may be measured in patients with abnormal calcium levels or kidney problems.

Taking a Vitamin D Test

A blood sample or blood test at home is used to measure your vitamin D level as ordered by a health care provider. A blood sample will usually be taken from your arm in a medical setting like a doctor’s office or hospital and analyzed in a medical laboratory.

Before the test

Usually, there are no special preparations required for vitamin D blood testing. Your healthcare provider will tell you if any preparations are needed for your test.

During the test

Most often, a small blood sample is taken from a vein in your arm. The healthcare professional taking the sample may tie a band around your upper arm to make your veins easier to locate. Then they disinfect a small patch of skin with an antiseptic wipe. Next, they insert a needle attached to a collection tube into your skin and withdraw a small amount of blood.

After the test

After your blood sample has been collected and the needle is withdrawn, you may be asked to apply pressure to the site with a piece of gauze and then a bandage. This stops the site from bleeding and may prevent bruising. You will be able to resume your normal activities, including driving, immediately after the test.

There are very few risks associated with a blood draw. You may have slight bruising in the area where the blood sample was taken, especially if you do not maintain pressure on the wound for several minutes.

Vitamin D Test Results

Receiving test results

Vitamin D blood test results are usually available within a few business days. Your healthcare provider may call you with the results or request that you make an appointment to discuss them. You may also receive your results through a secure online patient portal or in the mail.

Interpreting test results

Your test report will include information about your level of vitamin D and the reference range used to interpret your result. Reference ranges are the test result values that are considered optimal for health. Results that fall outside the reference range may indicate a health issue.

Reference ranges can vary by laboratory. Additionally, some labs may break down your levels of vitamin D2 and D3, while other laboratories report the combined total.

Medical experts and organizations have differing opinions on the ideal levels of vitamin D. Generally, test results can be used to distinguish between an insufficiency, in which vitamin D amounts are only slightly outside of the ideal range, and a deficiency, which can cause more serious problems.

The table below summarizes the test results, interpretations, and potential medical outcomes that experts consider when looking at a patient’s 25-hydroxyvitamin D level:

Test Result Interpretation Potential Medical Outcome
Deficient Vitamin D levels too low to support healthy bodily functions There is not enough calcium in the blood. Weakening of bones or painful bone problems may occur.
Insufficient Vitamin D levels lower than the ideal reference range Most people are asymptomatic. There may be accelerated bone loss or increased risk of fracture.
Sufficient Vitamin D levels fall within reference range There is an adequate amount of vitamin D and no known risks for health problems.
Risk of toxicity Excessive amount of vitamin D in the body This can cause too much calcium to circulate in the blood, which can lead to confusion, loss of appetite, vomiting, and muscle weakness.

Questions for your doctor about test results

You may wish to ask your doctor or other health care provider questions about your vitamin D test results, such as:

  • Do my vitamin D test levels indicate a deficiency or insufficiency?
  • Do my test results show that I have been taking too much vitamin D as a supplement?
  • Will additional testing be needed?
  • Do my test results indicate that treatment is needed?

Why Sunrise Diagnostic Centre?

Sunrise Diagnostic Centre (A Unit of Aadishakti Healthcare Pvt. Ltd.) is an integrated healthcare model, providing multiple healthcare services under one roof, in a state-of-the-art facility, equipped with ultra-advanced medical equipment with cutting-edge technology.

Sunrise Diagnostic Centre is NABL and ISO Certified & Trusted by 122 Doctors & 30+ Hospitals, We offer a comprehensive range of 4000+ clinical laboratory tests and Home Collection Services in Pune. which are used for prediction, early detection, diagnostic screening, confirmation and/or monitoring of the disease.

Book a blood test or expert health check-up package online with Sunrise Diagnostic Centre-

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Frequently Asked Questions

What happens if you take vitamin D on an empty stomach?2022-12-07T07:04:54+00:00

A study found that 32% more Vitamin D3 is absorbed by the body when it is taken with a meal. It is also important to note that taking any kind of supplement (not just fat-soluble ones) on an empty stomach causes some people gastrointestinal distress, including nausea or vomiting.

What if Results are Present/ Absent?2022-12-07T07:05:05+00:00

Low levels of Vitamin D are associated with osteoporosis, bone weakness and increased chance of fractures. Severe deficiency can lead to Rickets and osteocalcin.
Very high levels are associated with nausea, vomiting, poor appetite, constipation, weakness

What to Expect During Vitamin D Blood Test?2022-12-07T07:05:14+00:00

During a vitamin d blood test, a health care professional will take a blood sample from a vein in your arm, using a small needle. After the needle is inserted, a small amount of blood will be collected into a test tube or vial. You may feel a little sting when the needle goes in or out. This usually takes less than five minutes.
Low vitamin D levels can help diagnose a case of secondary hyperparathyroidism.
Often Vitamin D levels are tested along with serum calcium, phosphorous, parathyroid hormone.
For patients with renal diseases 1,25 dihydroxy vitamin D should be tested.

What Does Vitamin D Blood Test Measure?2022-12-07T07:05:22+00:00

Vitamin d blood test measures level of 25 hydroxy vitamin D total.

Why is Vitamin D Blood Test Done?2022-12-07T07:05:32+00:00

Vitamin d blood test is done to measure Vitamin D levels in body.
Testing is required to diagnose rickets in children and osteomalacia in adults which presents as bone deformity and increase risk of fracture. Levels are also estimated to monitor vitamin D replacement therapy.

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